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Mikrobiologie
Nächste Ebene öffnen
Team
Lehre
Nächste Ebene öffnen
Bachelor
Master
Promotion (Ph D)
Forschung
Nächste Ebene öffnen
Zellbiologie
Mikrobielle Interaktionen
Biotechnologie
Publikationen
Leitung Institut
Mehr
Nächste Ebene öffnen
Netzwerk
Aktuelle Informationen der Mikrobiologie
Service
Kontakt
Ustilago community
Nächste Ebene öffnen
Types of genetic engeneering
Nächste Ebene öffnen
1. Knock out
Nächste Ebene öffnen
pMF1-c with carboxin resistance cassette
pMF1-hs with hygromycin resistance cassette
pMF1-n with nourseothricin resistance cassette
pMF1-p with phleomycin resistance cassette
pMF1-g with geneticin resistance cassette
1.1 Knock out with recycling of resistance marker
Nächste Ebene öffnen
pMF1-1hs1 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs2 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs3 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs4 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs5 with hygromycin resistence cassette for subsequent recycling
2.1 Knock in with regulated crg1 promoter
Nächste Ebene öffnen
pMF2-1c with carboxin resistance cassette
pMF2-1h with hygromycin resistance cassette
pMF2-1n with nourseothricin resistance cassette
pMF2-1p with phleomycin resistance cassette
pMF2-1g with geneticin resistance cassette
2.2 Knock in with regulated nar1 promoter
Nächste Ebene öffnen
pMF2-2c with carboxin resistance cassette
pMF2-2h with hygromycin resistance cassette
pMF2-2n with nourseothricin resistance cassette
pMF2-2p with phleomycin resistance cassette
pMF2-2g with geneticin resistance cassette
2.3 Knock in with constitutive otef promoter
Nächste Ebene öffnen
pMF2-3c with carboxin resistance cassette
pMF2-3h with hygromycin resistance cassette
pMF2-3n with nourseothricin resistance cassette
pMF2-3p with phleomycin resistance cassette
pMF2-3g with geneticin resistance cassette
2.4 Knock in with regulated tetracycline promoter
Nächste Ebene öffnen
pMF2-4c with carboxin resistance cassette
pMF2-4h with hygromycin resistance cassette
pMF2-4n with nourseothricin resistance cassette
pMF2-4p with phleomycin resistance cassette
pMF2-4g with geneticin resistance cassette
3. Knock in with gfp transciptonally
Nächste Ebene öffnen
pMF3-c with carboxin resistance cassette
pMF3-h with hygromycin resistance cassette
pMF3-n with nourseothricin resistance cassette
pMF3-p with phleomycin resistance cassette
pMF3-g with geneticin resistance cassette
4.1 Knock in with regulated crg1 promoter & Gfp as reporter gene
Nächste Ebene öffnen
pMF4-1c with carboxin resistance cassette
pMF4-1h with hygromycin resistance cassette
pMF4-1n with nourseothricin resistance cassette
pMF4-1p with phleomycin resistance cassette
pMF4-1g with geneticin resistance cassette
4.2 Knock in with regulated nar1 promoter & Gfp as reporter gene
Nächste Ebene öffnen
pMF4-2c with carboxin resistance cassette
pMF4-2h with hygromycin resistance cassette
pMF4-2n with nourseothricin resistance cassette
pMF4-2p with phleomycin resistance cassette
pMF4-2g with geneticin resistance cassette
4.3 Knock in with constitutiv otef promoter & Gfp as reporter gene
Nächste Ebene öffnen
pMF4-3c with carboxin resistance cassette
pMF4-3h with hygromycin resistance cassette
pMF4-3n with nourseothricin resistance cassette
pMF4-3p with phleomycin resistance cassette
pMF4-3g with geneticin resistance cassette
5.1 C-terminal fusion with enhanced version of Gfp
Nächste Ebene öffnen
pMF5-1c with carboxin resistance cassette
pMF5-1h with hygromycin resistance cassette
pMF5-1n with nourseothricin resistance cassette
pMF5-1p with phleomycin resistance cassette
pMF5-1g with geneticin resistance cassette
5.2 C-terminal fusion with monomeric Rfp
Nächste Ebene öffnen
pMF5-2c with carboxin resistance cassette
pMF5-2h with hygromycin resistance cassette
pMF5-2n with nourseothricin resistance cassette
pMF5-2p with phleomycin resistance cassette
pMF5-2g with geneticin resistance cassette
5.3 C-terminal fusion with Gfp TapTag
Nächste Ebene öffnen
pMF5-3c with carboxin resistance cassette
pMF5-3h with hygromycin resistance cassette
pMF5-3n with nourseothricin resistance cassette
pMF5-3p with phleomycin resistance cassette
pMF5-3g with geneticin resistance cassette
5.4 C-terminal fusion with triple Gfp
Nächste Ebene öffnen
pMF5-4c with carboxin resistance cassette
pMF5-4h with hygromycin resistance cassette
pMF5-4n with nourseothricin resistance cassette
pMF5-4p with phleomycin resistance cassette
pMF5-4g with geneticin resistance cassette
5.5 C-terminal fusion with mCherry and triple Myc
Nächste Ebene öffnen
pMF5-5c with carboxin resistance cassette
pMF5-5h with hygromycin resistance cassette
pMF5-5n with nourseothricin resistance cassette
pMF5-5p with phleomycin resistance cassette
pMF5-5g with geneticin resistance cassette
5.6 C-terminal fusion with Yfp
Nächste Ebene öffnen
pMF5-6c with carboxin resistance cassette
pMF5-6h with hygromycin resistance cassette
pMF5-6n with nourseothricin resistance cassette
pMF5-6p with phleomycin resistance cassette
pMF5-6g with geneticin resistance cassette
5.7 C-terminal fusion with gfp, TapTag (ctt), his10
Nächste Ebene öffnen
pMF5-7c with carboxin resistance cassette
pMF5-7h with hygromycin resistance cassette
pMF5-7n with nourseothricin resistance cassette
pMF5-7p with phleomycin resistance cassette
pMF5-7g with geneticin resistance cassette
5.8 C-terminal fusion with TapTag (ctt; fusion)
Nächste Ebene öffnen
pMF5-8c with carboxin resistance cassette
pMF5-8h with hygromycin resistance cassette
pMF5-8n with nourseothricin resistance cassette
pMF5-8p with phleomycin resistance cassette
pMF5-8g with geneticin resistance cassette
5.9 C-terminal fusion with triple HA (fusion)
Nächste Ebene öffnen
pMF5-9c with carboxin resistance cassette
pMF5-9h with hygromycin resistance cassette
pMF5-9n with nourseothricin resistance cassette
pMF5-9p with phleomycin resistance cassette
pMF5-9g with geneticin resistance cassette
5.10 C-terminal fusion with triple Myc
Nächste Ebene öffnen
pMF5-10c with carboxin resistance cassette
pMF5-10h with hygromycin resistance cassette
pMF5-10n with nourseothricin resistance cassette
pMF5-10p with phleomycin resistance cassette
pMF5-10g with geneticin resistance cassette
5.11 C-terminal fusion with triple FLAG
Nächste Ebene öffnen
pMF5-11c with carboxin resistance cassette
pMF5-11h with hygromycin resistance cassette
pMF5-11n with nourseothricin resistance cassette
pMF5-11p with phleomycin resistance cassette
pMF5-11g with geneticin resistance cassette
5.12 C-terminal fusion with Cfp
Nächste Ebene öffnen
pMF5-12c with carboxin resistance cassette
pMF5-12h with hygromycin resistance cassette
pMF5-12n with nourseothricin resistance cassette
pMF5-12p with phleomycin resistance cassette
pMF5-12g with geneticin resistance cassette
5.13 C-terminal fusion with triple Cherry
Nächste Ebene öffnen
pMF5-13c with carboxin resistance cassette
pMF5-13h with hygromycin resistance cassette
pMF5-13n with nourseothricin resistance cassette
pMF5-13p with phleomycin resistance cassette
pMF5-13g with geneticin resistance cassette
5.15 C-terminal fusion with mcherry
Nächste Ebene öffnen
pMF5-15c with carboxin resistance cassette
pMF5-15h with hygromycin resistance cassette
pMF5-15n with nourseothricin resistance cassette
pMF5-15p with phleomycin resistance cassette
pMF5-15g with geneticin resistance cassette
Miscellaneous
Nächste Ebene öffnen
pFLPexpC for expression of FLP recombinase under control of the crg1 promoter
Golden Gate cloning system
Nächste Ebene öffnen
0. Basic vectors
Nächste Ebene öffnen
0.1 pDest
0.2 pStorI
0.3 pStorII
1. Knock out
Nächste Ebene öffnen
pStorI-1c
pStorI-1hs
pStorI-1n
pStorI-1p
pStorI-1g
1.1 Knock out with recycling (under construction)
Nächste Ebene öffnen
pStoreI-1rh
2.3 knockin with constitutive active o2tef promoter (under construction)
Nächste Ebene öffnen
pStorI-2-3h
List of Scientists that obtained plasmids
List of Scientists that contributed plasmids
Table listing
Mikrobiologie
Menü schließen
Zur Übersichtsseite Mikrobiologie
Team
Lehre
Nächste Ebene öffnen
Nächste Ebene schließen
Bachelor
Master
Promotion (Ph D)
Forschung
Nächste Ebene öffnen
Nächste Ebene schließen
Zellbiologie
Mikrobielle Interaktionen
Biotechnologie
Publikationen
Leitung Institut
Mehr
Nächste Ebene öffnen
Nächste Ebene schließen
Netzwerk
Aktuelle Informationen der Mikrobiologie
Service
Kontakt
Ustilago community
Nächste Ebene öffnen
Nächste Ebene schließen
Types of genetic engeneering
Nächste Ebene öffnen
Nächste Ebene schließen
1. Knock out
Nächste Ebene öffnen
Nächste Ebene schließen
pMF1-c with carboxin resistance cassette
pMF1-hs with hygromycin resistance cassette
pMF1-n with nourseothricin resistance cassette
pMF1-p with phleomycin resistance cassette
pMF1-g with geneticin resistance cassette
1.1 Knock out with recycling of resistance marker
Nächste Ebene öffnen
Nächste Ebene schließen
pMF1-1hs1 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs2 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs3 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs4 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs5 with hygromycin resistence cassette for subsequent recycling
2.1 Knock in with regulated crg1 promoter
Nächste Ebene öffnen
Nächste Ebene schließen
pMF2-1c with carboxin resistance cassette
pMF2-1h with hygromycin resistance cassette
pMF2-1n with nourseothricin resistance cassette
pMF2-1p with phleomycin resistance cassette
pMF2-1g with geneticin resistance cassette
2.2 Knock in with regulated nar1 promoter
Nächste Ebene öffnen
Nächste Ebene schließen
pMF2-2c with carboxin resistance cassette
pMF2-2h with hygromycin resistance cassette
pMF2-2n with nourseothricin resistance cassette
pMF2-2p with phleomycin resistance cassette
pMF2-2g with geneticin resistance cassette
2.3 Knock in with constitutive otef promoter
Nächste Ebene öffnen
Nächste Ebene schließen
pMF2-3c with carboxin resistance cassette
pMF2-3h with hygromycin resistance cassette
pMF2-3n with nourseothricin resistance cassette
pMF2-3p with phleomycin resistance cassette
pMF2-3g with geneticin resistance cassette
2.4 Knock in with regulated tetracycline promoter
Nächste Ebene öffnen
Nächste Ebene schließen
pMF2-4c with carboxin resistance cassette
pMF2-4h with hygromycin resistance cassette
pMF2-4n with nourseothricin resistance cassette
pMF2-4p with phleomycin resistance cassette
pMF2-4g with geneticin resistance cassette
3. Knock in with gfp transciptonally
Nächste Ebene öffnen
Nächste Ebene schließen
pMF3-c with carboxin resistance cassette
pMF3-h with hygromycin resistance cassette
pMF3-n with nourseothricin resistance cassette
pMF3-p with phleomycin resistance cassette
pMF3-g with geneticin resistance cassette
4.1 Knock in with regulated crg1 promoter & Gfp as reporter gene
Nächste Ebene öffnen
Nächste Ebene schließen
pMF4-1c with carboxin resistance cassette
pMF4-1h with hygromycin resistance cassette
pMF4-1n with nourseothricin resistance cassette
pMF4-1p with phleomycin resistance cassette
pMF4-1g with geneticin resistance cassette
4.2 Knock in with regulated nar1 promoter & Gfp as reporter gene
Nächste Ebene öffnen
Nächste Ebene schließen
pMF4-2c with carboxin resistance cassette
pMF4-2h with hygromycin resistance cassette
pMF4-2n with nourseothricin resistance cassette
pMF4-2p with phleomycin resistance cassette
pMF4-2g with geneticin resistance cassette
4.3 Knock in with constitutiv otef promoter & Gfp as reporter gene
Nächste Ebene öffnen
Nächste Ebene schließen
pMF4-3c with carboxin resistance cassette
pMF4-3h with hygromycin resistance cassette
pMF4-3n with nourseothricin resistance cassette
pMF4-3p with phleomycin resistance cassette
pMF4-3g with geneticin resistance cassette
5.1 C-terminal fusion with enhanced version of Gfp
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-1c with carboxin resistance cassette
pMF5-1h with hygromycin resistance cassette
pMF5-1n with nourseothricin resistance cassette
pMF5-1p with phleomycin resistance cassette
pMF5-1g with geneticin resistance cassette
5.2 C-terminal fusion with monomeric Rfp
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-2c with carboxin resistance cassette
pMF5-2h with hygromycin resistance cassette
pMF5-2n with nourseothricin resistance cassette
pMF5-2p with phleomycin resistance cassette
pMF5-2g with geneticin resistance cassette
5.3 C-terminal fusion with Gfp TapTag
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-3c with carboxin resistance cassette
pMF5-3h with hygromycin resistance cassette
pMF5-3n with nourseothricin resistance cassette
pMF5-3p with phleomycin resistance cassette
pMF5-3g with geneticin resistance cassette
5.4 C-terminal fusion with triple Gfp
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-4c with carboxin resistance cassette
pMF5-4h with hygromycin resistance cassette
pMF5-4n with nourseothricin resistance cassette
pMF5-4p with phleomycin resistance cassette
pMF5-4g with geneticin resistance cassette
5.5 C-terminal fusion with mCherry and triple Myc
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-5c with carboxin resistance cassette
pMF5-5h with hygromycin resistance cassette
pMF5-5n with nourseothricin resistance cassette
pMF5-5p with phleomycin resistance cassette
pMF5-5g with geneticin resistance cassette
5.6 C-terminal fusion with Yfp
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-6c with carboxin resistance cassette
pMF5-6h with hygromycin resistance cassette
pMF5-6n with nourseothricin resistance cassette
pMF5-6p with phleomycin resistance cassette
pMF5-6g with geneticin resistance cassette
5.7 C-terminal fusion with gfp, TapTag (ctt), his10
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-7c with carboxin resistance cassette
pMF5-7h with hygromycin resistance cassette
pMF5-7n with nourseothricin resistance cassette
pMF5-7p with phleomycin resistance cassette
pMF5-7g with geneticin resistance cassette
5.8 C-terminal fusion with TapTag (ctt; fusion)
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-8c with carboxin resistance cassette
pMF5-8h with hygromycin resistance cassette
pMF5-8n with nourseothricin resistance cassette
pMF5-8p with phleomycin resistance cassette
pMF5-8g with geneticin resistance cassette
5.9 C-terminal fusion with triple HA (fusion)
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-9c with carboxin resistance cassette
pMF5-9h with hygromycin resistance cassette
pMF5-9n with nourseothricin resistance cassette
pMF5-9p with phleomycin resistance cassette
pMF5-9g with geneticin resistance cassette
5.10 C-terminal fusion with triple Myc
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-10c with carboxin resistance cassette
pMF5-10h with hygromycin resistance cassette
pMF5-10n with nourseothricin resistance cassette
pMF5-10p with phleomycin resistance cassette
pMF5-10g with geneticin resistance cassette
5.11 C-terminal fusion with triple FLAG
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-11c with carboxin resistance cassette
pMF5-11h with hygromycin resistance cassette
pMF5-11n with nourseothricin resistance cassette
pMF5-11p with phleomycin resistance cassette
pMF5-11g with geneticin resistance cassette
5.12 C-terminal fusion with Cfp
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-12c with carboxin resistance cassette
pMF5-12h with hygromycin resistance cassette
pMF5-12n with nourseothricin resistance cassette
pMF5-12p with phleomycin resistance cassette
pMF5-12g with geneticin resistance cassette
5.13 C-terminal fusion with triple Cherry
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-13c with carboxin resistance cassette
pMF5-13h with hygromycin resistance cassette
pMF5-13n with nourseothricin resistance cassette
pMF5-13p with phleomycin resistance cassette
pMF5-13g with geneticin resistance cassette
5.15 C-terminal fusion with mcherry
Nächste Ebene öffnen
Nächste Ebene schließen
pMF5-15c with carboxin resistance cassette
pMF5-15h with hygromycin resistance cassette
pMF5-15n with nourseothricin resistance cassette
pMF5-15p with phleomycin resistance cassette
pMF5-15g with geneticin resistance cassette
Miscellaneous
Nächste Ebene öffnen
Nächste Ebene schließen
pFLPexpC for expression of FLP recombinase under control of the crg1 promoter
Golden Gate cloning system
Nächste Ebene öffnen
Nächste Ebene schließen
0. Basic vectors
Nächste Ebene öffnen
Nächste Ebene schließen
0.1 pDest
0.2 pStorI
0.3 pStorII
1. Knock out
Nächste Ebene öffnen
Nächste Ebene schließen
pStorI-1c
pStorI-1hs
pStorI-1n
pStorI-1p
pStorI-1g
1.1 Knock out with recycling (under construction)
Nächste Ebene öffnen
Nächste Ebene schließen
pStoreI-1rh
2.3 knockin with constitutive active o2tef promoter (under construction)
Nächste Ebene öffnen
Nächste Ebene schließen
pStorI-2-3h
List of Scientists that obtained plasmids
List of Scientists that contributed plasmids
Table listing
HHU
Math.-Nat. Fakultät
Biologie
Mikrobiologie
Mehr
Ustilago community
Types of genetic engeneering
5.7 C-terminal fusion with gfp, TapTag (ctt), his10
pMF5-7c with carboxin resistance cassette
pMF5-7c with carboxin resistance cassette
Verantwortlichkeit:
Prof. Dr. Michael Feldbrügge
: Per E-Mail kontaktieren
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