Types of genetic engeneering
- 1. Knock out
- 1.1 Knock out with recycling of resistance marker
- 2.1 Knock in with regulated crg1 promoter
- 2.2 Knock in with regulated nar1 promoter
- 2.3 Knock in with constitutive otef promoter
- 2.4 Knock in with regulated tetracycline promoter
- 3. Knock in with gfp transciptonally
- 4.1 Knock in with regulated crg1 promoter & Gfp as reporter gene
- 4.2 Knock in with regulated nar1 promoter & Gfp as reporter gene
- 4.3 Knock in with constitutiv otef promoter & Gfp as reporter gene
- 5.1 C-terminal fusion with enhanced version of Gfp
- 5.2 C-terminal fusion with monomeric Rfp
- 5.3 C-terminal fusion with Gfp TapTag
- 5.4 C-terminal fusion with triple Gfp
- 5.5 C-terminal fusion with mCherry and triple Myc
- 5.6 C-terminal fusion with Yfp
- 5.7 C-terminal fusion with gfp, TapTag (ctt), his10
- 5.8 C-terminal fusion with TapTag (ctt; fusion)
- 5.9 C-terminal fusion with triple HA (fusion)
- 5.10 C-terminal fusion with triple Myc
- 5.11 C-terminal fusion with triple FLAG
- 5.12 C-terminal fusion with Cfp
- 5.13 C-terminal fusion with triple Cherry
- 5.15 C-terminal fusion with mcherry
