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Microbiology
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Cell Biology
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Ustilago community
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Types of genetic engeneering
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1. Knock out
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pMF1-c with carboxin resistance cassette
pMF1-hs with hygromycin resistance cassette
pMF1-n with nourseothricin resistance cassette
pMF1-p with phleomycin resistance cassette
pMF1-g with geneticin resistance cassette
1.1 Knock out with recycling of resistance marker
Open next level
pMF1-1hs1 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs2 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs3 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs4 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs5 with hygromycin resistence cassette for subsequent recycling
2.1 Knock in with regulated crg1 promoter
Open next level
pMF2-1c with carboxin resistance cassette
pMF2-1h with hygromycin resistance cassette
pMF2-1n with nourseothricin resistance cassette
pMF2-1p with phleomycin resistance cassette
pMF2-1g with geneticin resistance cassette
2.2 Knock in with regulated nar1 promoter
Open next level
pMF2-2c with carboxin resistance cassette
pMF2-2h with hygromycin resistance cassette
pMF2-2n with nourseothricin resistance cassette
pMF2-2p with phleomycin resistance cassette
pMF2-2g with geneticin resistance cassette
2.3 Knock in with constitutive otef promoter
Open next level
pMF2-3c with carboxin resistance cassette
pMF2-3h with hygromycin resistance cassette
pMF2-3n with nourseothricin resistance cassette
pMF2-3p with phleomycin resistance cassette
pMF2-3g with geneticin resistance cassette
2.4 Knock in with regulated tetracycline promoter
Open next level
pMF2-4c with carboxin resistance cassette
pMF2-4h with hygromycin resistance cassette
pMF2-4n with nourseothricin resistance cassette
pMF2-4p with phleomycin resistance cassette
pMF2-4g with geneticin resistance cassette
3. Knock in with gfp transciptonally
Open next level
pMF3-c with carboxin resistance cassette
pMF3-h with hygromycin resistance cassette
pMF3-n with nourseothricin resistance cassette
pMF3-p with phleomycin resistance cassette
pMF3-g with geneticin resistance cassette
4.1 Knock in with regulated crg1 promoter & Gfp as reporter gene
Open next level
pMF4-1c with carboxin resistance cassette
pMF4-1h with hygromycin resistance cassette
pMF4-1n with nourseothricin resistance cassette
pMF4-1p with phleomycin resistance cassette
pMF4-1g with geneticin resistance cassette
4.2 Knock in with regulated nar1 promoter & Gfp as reporter gene
Open next level
pMF4-2c with carboxin resistance cassette
pMF4-2h with hygromycin resistance cassette
pMF4-2n with nourseothricin resistance cassette
pMF4-2p with phleomycin resistance cassette
pMF4-2g with geneticin resistance cassette
4.3 Knock in with constitutiv otef promoter & Gfp as reporter gene
Open next level
pMF4-3c with carboxin resistance cassette
pMF4-3h with hygromycin resistance cassette
pMF4-3n with nourseothricin resistance cassette
pMF4-3p with phleomycin resistance cassette
pMF4-3g with geneticin resistance cassette
5.1 C-terminal fusion with enhanced version of Gfp
Open next level
pMF5-1c with carboxin resistance cassette
pMF5-1h with hygromycin resistance cassette
pMF5-1n with nourseothricin resistance cassette
pMF5-1p with phleomycin resistance cassette
pMF5-1g with geneticin resistance cassette
5.2 C-terminal fusion with monomeric Rfp
Open next level
pMF5-2c with carboxin resistance cassette
pMF5-2h with hygromycin resistance cassette
pMF5-2n with nourseothricin resistance cassette
pMF5-2p with phleomycin resistance cassette
pMF5-2g with geneticin resistance cassette
5.3 C-terminal fusion with Gfp TapTag
Open next level
pMF5-3c with carboxin resistance cassette
pMF5-3h with hygromycin resistance cassette
pMF5-3n with nourseothricin resistance cassette
pMF5-3p with phleomycin resistance cassette
pMF5-3g with geneticin resistance cassette
5.4 C-terminal fusion with triple Gfp
Open next level
pMF5-4c with carboxin resistance cassette
pMF5-4h with hygromycin resistance cassette
pMF5-4n with nourseothricin resistance cassette
pMF5-4p with phleomycin resistance cassette
pMF5-4g with geneticin resistance cassette
5.5 C-terminal fusion with mCherry and triple Myc
Open next level
pMF5-5c with carboxin resistance cassette
pMF5-5h with hygromycin resistance cassette
pMF5-5n with nourseothricin resistance cassette
pMF5-5p with phleomycin resistance cassette
pMF5-5g with geneticin resistance cassette
5.6 C-terminal fusion with Yfp
Open next level
pMF5-6c with carboxin resistance cassette
pMF5-6h with hygromycin resistance cassette
pMF5-6n with nourseothricin resistance cassette
pMF5-6p with phleomycin resistance cassette
pMF5-6g with geneticin resistance cassette
5.7 C-terminal fusion with gfp, TapTag (ctt), his10
Open next level
pMF5-7c with carboxin resistance cassette
pMF5-7h with hygromycin resistance cassette
pMF5-7n with nourseothricin resistance cassette
pMF5-7p with phleomycin resistance cassette
pMF5-7g with geneticin resistance cassette
5.8 C-terminal fusion with TapTag (ctt; fusion)
Open next level
pMF5-8c with carboxin resistance cassette
pMF5-8h with hygromycin resistance cassette
pMF5-8n with nourseothricin resistance cassette
pMF5-8p with phleomycin resistance cassette
pMF5-8g with geneticin resistance cassette
5.9 C-terminal fusion with triple HA (fusion)
Open next level
pMF5-9c with carboxin resistance cassette
pMF5-9h with hygromycin resistance cassette
pMF5-9n with nourseothricin resistance cassette
pMF5-9p with phleomycin resistance cassette
pMF5-9g with geneticin resistance cassette
5.10 C-terminal fusion with triple Myc
Open next level
pMF5-10c with carboxin resistance cassette
pMF5-10h with hygromycin resistance cassette
pMF5-10n with nourseothricin resistance cassette
pMF5-10p with phleomycin resistance cassette
pMF5-10g with geneticin resistance cassette
5.11 C-terminal fusion with triple FLAG
Open next level
pMF5-11c with carboxin resistance cassette
pMF5-11h with hygromycin resistance cassette
pMF5-11n with nourseothricin resistance cassette
pMF5-11p with phleomycin resistance cassette
pMF5-11g with geneticin resistance cassette
5.12 C-terminal fusion with Cfp
Open next level
pMF5-12c with carboxin resistance cassette
pMF5-12h with hygromycin resistance cassette
pMF5-12n with nourseothricin resistance cassette
pMF5-12p with phleomycin resistance cassette
pMF5-12g with geneticin resistance cassette
5.13 C-terminal fusion with triple Cherry
Open next level
pMF5-13c with carboxin resistance cassette
pMF5-13h with hygromycin resistance cassette
pMF5-13n with nourseothricin resistance cassette
pMF5-13p with phleomycin resistance cassette
pMF5-13g with geneticin resistance cassette
5.15 C-terminal fusion with mcherry
Open next level
pMF5-15c with carboxin resistance cassette
pMF5-15h with hygromycin resistance cassette
pMF5-15n with nourseothricin resistance cassette
pMF5-15p with phleomycin resistance cassette
pMF5-15g with geneticin resistance cassette
Miscellaneous
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pFLPexpC for expression of FLP recombinase under control of the crg1 promoter
Golden Gate cloning system
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0. Basic vectors
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0.1 pDest
0.2 pStorI
0.3 pStorII
1. Knock out
Open next level
pStorI-1c
pStorI-1hs
pStorI-1n
pStorI-1p
pStorI-1g
1.1 Knock out with recycling (under construction)
Open next level
pStoreI-1rh
2.3 knockin with constitutive active o2tef promoter (under construction)
Open next level
pStorI-2-3h
List of Scientists that obtained plasmids
List of Scientists that contributed plasmids
Table listing
Microbiology
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To homepage Microbiology
Team
Teaching
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Bachelor
Master
Promotion (Ph D)
Research
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Cell Biology
Microbial Interactions
Biotechnology
Publications
Head
More
Open next level
Close next level
Collaborations
News
Service
Kontakt
Ustilago community
Open next level
Close next level
Types of genetic engeneering
Open next level
Close next level
1. Knock out
Open next level
Close next level
pMF1-c with carboxin resistance cassette
pMF1-hs with hygromycin resistance cassette
pMF1-n with nourseothricin resistance cassette
pMF1-p with phleomycin resistance cassette
pMF1-g with geneticin resistance cassette
1.1 Knock out with recycling of resistance marker
Open next level
Close next level
pMF1-1hs1 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs2 with hygromycin resistance cassette for subsequent recycling
pMF1-1hs3 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs4 with hygromycin resistence cassette for subsequent recycling
pMF1-1hs5 with hygromycin resistence cassette for subsequent recycling
2.1 Knock in with regulated crg1 promoter
Open next level
Close next level
pMF2-1c with carboxin resistance cassette
pMF2-1h with hygromycin resistance cassette
pMF2-1n with nourseothricin resistance cassette
pMF2-1p with phleomycin resistance cassette
pMF2-1g with geneticin resistance cassette
2.2 Knock in with regulated nar1 promoter
Open next level
Close next level
pMF2-2c with carboxin resistance cassette
pMF2-2h with hygromycin resistance cassette
pMF2-2n with nourseothricin resistance cassette
pMF2-2p with phleomycin resistance cassette
pMF2-2g with geneticin resistance cassette
2.3 Knock in with constitutive otef promoter
Open next level
Close next level
pMF2-3c with carboxin resistance cassette
pMF2-3h with hygromycin resistance cassette
pMF2-3n with nourseothricin resistance cassette
pMF2-3p with phleomycin resistance cassette
pMF2-3g with geneticin resistance cassette
2.4 Knock in with regulated tetracycline promoter
Open next level
Close next level
pMF2-4c with carboxin resistance cassette
pMF2-4h with hygromycin resistance cassette
pMF2-4n with nourseothricin resistance cassette
pMF2-4p with phleomycin resistance cassette
pMF2-4g with geneticin resistance cassette
3. Knock in with gfp transciptonally
Open next level
Close next level
pMF3-c with carboxin resistance cassette
pMF3-h with hygromycin resistance cassette
pMF3-n with nourseothricin resistance cassette
pMF3-p with phleomycin resistance cassette
pMF3-g with geneticin resistance cassette
4.1 Knock in with regulated crg1 promoter & Gfp as reporter gene
Open next level
Close next level
pMF4-1c with carboxin resistance cassette
pMF4-1h with hygromycin resistance cassette
pMF4-1n with nourseothricin resistance cassette
pMF4-1p with phleomycin resistance cassette
pMF4-1g with geneticin resistance cassette
4.2 Knock in with regulated nar1 promoter & Gfp as reporter gene
Open next level
Close next level
pMF4-2c with carboxin resistance cassette
pMF4-2h with hygromycin resistance cassette
pMF4-2n with nourseothricin resistance cassette
pMF4-2p with phleomycin resistance cassette
pMF4-2g with geneticin resistance cassette
4.3 Knock in with constitutiv otef promoter & Gfp as reporter gene
Open next level
Close next level
pMF4-3c with carboxin resistance cassette
pMF4-3h with hygromycin resistance cassette
pMF4-3n with nourseothricin resistance cassette
pMF4-3p with phleomycin resistance cassette
pMF4-3g with geneticin resistance cassette
5.1 C-terminal fusion with enhanced version of Gfp
Open next level
Close next level
pMF5-1c with carboxin resistance cassette
pMF5-1h with hygromycin resistance cassette
pMF5-1n with nourseothricin resistance cassette
pMF5-1p with phleomycin resistance cassette
pMF5-1g with geneticin resistance cassette
5.2 C-terminal fusion with monomeric Rfp
Open next level
Close next level
pMF5-2c with carboxin resistance cassette
pMF5-2h with hygromycin resistance cassette
pMF5-2n with nourseothricin resistance cassette
pMF5-2p with phleomycin resistance cassette
pMF5-2g with geneticin resistance cassette
5.3 C-terminal fusion with Gfp TapTag
Open next level
Close next level
pMF5-3c with carboxin resistance cassette
pMF5-3h with hygromycin resistance cassette
pMF5-3n with nourseothricin resistance cassette
pMF5-3p with phleomycin resistance cassette
pMF5-3g with geneticin resistance cassette
5.4 C-terminal fusion with triple Gfp
Open next level
Close next level
pMF5-4c with carboxin resistance cassette
pMF5-4h with hygromycin resistance cassette
pMF5-4n with nourseothricin resistance cassette
pMF5-4p with phleomycin resistance cassette
pMF5-4g with geneticin resistance cassette
5.5 C-terminal fusion with mCherry and triple Myc
Open next level
Close next level
pMF5-5c with carboxin resistance cassette
pMF5-5h with hygromycin resistance cassette
pMF5-5n with nourseothricin resistance cassette
pMF5-5p with phleomycin resistance cassette
pMF5-5g with geneticin resistance cassette
5.6 C-terminal fusion with Yfp
Open next level
Close next level
pMF5-6c with carboxin resistance cassette
pMF5-6h with hygromycin resistance cassette
pMF5-6n with nourseothricin resistance cassette
pMF5-6p with phleomycin resistance cassette
pMF5-6g with geneticin resistance cassette
5.7 C-terminal fusion with gfp, TapTag (ctt), his10
Open next level
Close next level
pMF5-7c with carboxin resistance cassette
pMF5-7h with hygromycin resistance cassette
pMF5-7n with nourseothricin resistance cassette
pMF5-7p with phleomycin resistance cassette
pMF5-7g with geneticin resistance cassette
5.8 C-terminal fusion with TapTag (ctt; fusion)
Open next level
Close next level
pMF5-8c with carboxin resistance cassette
pMF5-8h with hygromycin resistance cassette
pMF5-8n with nourseothricin resistance cassette
pMF5-8p with phleomycin resistance cassette
pMF5-8g with geneticin resistance cassette
5.9 C-terminal fusion with triple HA (fusion)
Open next level
Close next level
pMF5-9c with carboxin resistance cassette
pMF5-9h with hygromycin resistance cassette
pMF5-9n with nourseothricin resistance cassette
pMF5-9p with phleomycin resistance cassette
pMF5-9g with geneticin resistance cassette
5.10 C-terminal fusion with triple Myc
Open next level
Close next level
pMF5-10c with carboxin resistance cassette
pMF5-10h with hygromycin resistance cassette
pMF5-10n with nourseothricin resistance cassette
pMF5-10p with phleomycin resistance cassette
pMF5-10g with geneticin resistance cassette
5.11 C-terminal fusion with triple FLAG
Open next level
Close next level
pMF5-11c with carboxin resistance cassette
pMF5-11h with hygromycin resistance cassette
pMF5-11n with nourseothricin resistance cassette
pMF5-11p with phleomycin resistance cassette
pMF5-11g with geneticin resistance cassette
5.12 C-terminal fusion with Cfp
Open next level
Close next level
pMF5-12c with carboxin resistance cassette
pMF5-12h with hygromycin resistance cassette
pMF5-12n with nourseothricin resistance cassette
pMF5-12p with phleomycin resistance cassette
pMF5-12g with geneticin resistance cassette
5.13 C-terminal fusion with triple Cherry
Open next level
Close next level
pMF5-13c with carboxin resistance cassette
pMF5-13h with hygromycin resistance cassette
pMF5-13n with nourseothricin resistance cassette
pMF5-13p with phleomycin resistance cassette
pMF5-13g with geneticin resistance cassette
5.15 C-terminal fusion with mcherry
Open next level
Close next level
pMF5-15c with carboxin resistance cassette
pMF5-15h with hygromycin resistance cassette
pMF5-15n with nourseothricin resistance cassette
pMF5-15p with phleomycin resistance cassette
pMF5-15g with geneticin resistance cassette
Miscellaneous
Open next level
Close next level
pFLPexpC for expression of FLP recombinase under control of the crg1 promoter
Golden Gate cloning system
Open next level
Close next level
0. Basic vectors
Open next level
Close next level
0.1 pDest
0.2 pStorI
0.3 pStorII
1. Knock out
Open next level
Close next level
pStorI-1c
pStorI-1hs
pStorI-1n
pStorI-1p
pStorI-1g
1.1 Knock out with recycling (under construction)
Open next level
Close next level
pStoreI-1rh
2.3 knockin with constitutive active o2tef promoter (under construction)
Open next level
Close next level
pStorI-2-3h
List of Scientists that obtained plasmids
List of Scientists that contributed plasmids
Table listing
Home
Math.-Nat. Faculty
Biology
Microbiology
More
Ustilago community
Golden Gate cloning system
Golden Gate cloning system
Responsible for the content:
Prof. Dr. Michael Feldbrügge
: Contact by e-mail
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